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In this paper Mode-Matching Technique is implemented in the analysis of corrugated horn antenna. Furthermore, a new method for optimization of corrugated horn antennas is presented using Genetic Algorithm and the mode matching technique. Four corrugated horn antennas in X-band are designed using this approach. In three of the cases a corrugated horn antenna which was previously designed with conventional methods is optimized and good result was obtained. The results show the ability of genetic algorithm in design and optimization of corrugated horn antennas.